Any uncontrolled system incorporates variability in process and outcome particularly at the point of nucleation where the sample is most vulnerable. In order to process and store all samples in their optimum condition the critical points must be managed accurately and repeatably during each freezing process. LifestART Solutions has worked with many organisations and cell types to demonstrate the improved yield and quality of sample cryopreserved using controlled rate freezers.

Lifestart Solutions recommend the use of controlled rate freezers as part of any cryopreservation program.

The main advantages of using controlled rate freezing for cryopreservation are:

• Repeatable and quality controlled procedure

• Reduced risk of microbial contamination

• Reduced risk of cross contamination with other cell lines

• Reduced risk of genetic drift and morphological changes

• Work conducted using reference cells

• Reduced costs (consumables and staff time)